sirtuin 1  (Cell Signaling Technology Inc)

Journal: Anticancer research

Article Title: Antitumor Potential of Guttiferone E Combined With Carboplatin Against Osimertinib-resistant H1975 Lung Cancer Through Apoptosis

doi: 10.21873/anticanres.17248

Figure Lengend Snippet: Effect of guttiferone E, carboplatin and their combination (Combo) on xenotransplants in athymic nude mice. A: Representative western blots and quantification of poly-ADP ribose polymerase (PARP), sirtuin 1 (SIRT 1), sirtuin 7 (SIRT 7) and β-actin in tumor tissue lysate. B: Representative western blots and quantification of programmed death-ligand 1 (PD-L1), programmed death 1 (PD-1), mechanistic target of rapamycin (mTOR), superoxide dismutase (SOD2), and β-actin in the tumor tissue lysate. The values are expressed as the mean±standard deviation (n=3). Statistical significance is indicated as: *p<0.05, **p<0.01, ***p<0.001 vs. the control, and ^p<0.05, ^^p<0.01 and ^^^p<0.001 vs. single treatments. Otherwise, there is no significant difference between the treatments.

Article Snippet: Overnight incubation with primary antibodies specific to poly-ADP ribose polymerase (PARP) (cat no. 9542S), sirtuin 7 (SIRT7) (cat no. 5360S), sirtuin 1 (SIRT1) (cat no. 9475S), programmed death-ligand 1 (PD-L1) (cat no. 13684S), programmed death 1 (PD-1) (cat no. 86163S), mechanistic target of rapamycin (mTOR) (cat no. 2972S) (all at 1:1000 dilution; Cell Signaling Technology, Beverly, MA, USA), SOD2 (cat no. SC30080; Santa Cruz Biotechnology, Paso Robles, CA, USA), and β-actin (cat no. 4970L; 1:1000 dilution; Cell Signaling Technology) was performed.

Techniques: Western Blot, Standard Deviation, Control